mrc 1024 es confocal laser scanning microscope  (Bio-Rad)

Journal: Basic and Clinical Neuroscience

Article Title: Effect of Parenchymal Arachnoid on Brain Fluid Transport

doi: 10.32598/bcn.2022.3089.1

Figure Lengend Snippet: Brain slice cultured at 7 days with adding labelled arachnoid cells (red) Note: Nuclear staining is with DAPI (4′,6-diamidino-2-phenylindole). The image was taken at 100 μm depth using a BioRad MRC-1024 single photon confocal microscope 1024 merged with Brightfield (BioRad Cell Science, UK) (A taken at ×10 magnification, B taken at ×20 magnification).

Article Snippet: To determine if arachnoid cells cultured on brain slices survived and penetrated organotypic slices, 3 brain slices were fixed in 10% formalin at each time point (i.e. day 3, 7, and 10) and analyzed using a BioRad MRC-1024 single photon confocal microscope 1024 (BioRad Cell Science, UK).

Techniques: Slice Preparation, Cell Culture, Staining, Microscopy

Journal: Basic and Clinical Neuroscience

Article Title: Effect of Parenchymal Arachnoid on Brain Fluid Transport

doi: 10.32598/bcn.2022.3089.1

Figure Lengend Snippet: Brain slice cultured at 7 days with adding labelled arachnoid cells (red) including nuclear staining DAPI (4′,6-diamidino-2-phenylindole) (blue) A) The yellow outline indicates blood vessels within the brain slice, with the arachnoid growing nearby, the image was taken at ×40 magnification and 100 μm depth; B) Shows a brain slice cultured for 4 days, showing bipolar and tripolar morphology of arachnoid cells (red) Note: The image was taken at ×60 magnification and 50 μm depth using a BioRad MRC-1024 single photon confocal microscope 1024 (BioRad Cell Science, UK).

Article Snippet: To determine if arachnoid cells cultured on brain slices survived and penetrated organotypic slices, 3 brain slices were fixed in 10% formalin at each time point (i.e. day 3, 7, and 10) and analyzed using a BioRad MRC-1024 single photon confocal microscope 1024 (BioRad Cell Science, UK).

Techniques: Slice Preparation, Cell Culture, Staining, Microscopy

Journal: Basic and Clinical Neuroscience

Article Title: Effect of Parenchymal Arachnoid on Brain Fluid Transport

doi: 10.32598/bcn.2022.3089.1

Figure Lengend Snippet: Arachnoid (red) across the top line showing increased growth within brain slices over 10 days Note: Consistently low apoptosis (green) across the bottom row was recorded throughout the same growth period. The image was taken at 10× magnification at a 150 μm depth using a BioRad MRC-1024 single photon confocal microscope 1024 (BioRad Cell Science, UK).

Article Snippet: To determine if arachnoid cells cultured on brain slices survived and penetrated organotypic slices, 3 brain slices were fixed in 10% formalin at each time point (i.e. day 3, 7, and 10) and analyzed using a BioRad MRC-1024 single photon confocal microscope 1024 (BioRad Cell Science, UK).

Techniques: Microscopy

Journal: Basic and Clinical Neuroscience

Article Title: Effect of Parenchymal Arachnoid on Brain Fluid Transport

doi: 10.32598/bcn.2022.3089.1

Figure Lengend Snippet: Brain slices cultured for 7 days to monitor tight junction formation A) Claudin-5 staining (green) and labeled arachnoid (red), taken at 100 μm depth at ×10 magnification, B) X60 magnification using a BioRad MRC-1024 single photon confocal microscope 1024 (BioRad Cell Science, UK)

Article Snippet: To determine if arachnoid cells cultured on brain slices survived and penetrated organotypic slices, 3 brain slices were fixed in 10% formalin at each time point (i.e. day 3, 7, and 10) and analyzed using a BioRad MRC-1024 single photon confocal microscope 1024 (BioRad Cell Science, UK).

Techniques: Cell Culture, Staining, Labeling, Microscopy